Monday, July 14, 2008

toxin detection techniques

-Physicochemical Methods
In this method, the mycotoxin is extracted, purified, concentrated and separated via thin-layer chromatography before the mycotoxin in the food sample can be quantified.

-Immuno Assays
Mycotoxins are made to interact with specific antibodies before they are isolated. Radioimmunoassay (RIA) and enzyme-linked immunoabsorbent assay (ELISA) are two techniques of this method.

-Biological Assays
Biological systems such as cell and tissue cultures, animals and microorganisms are used as bioassays to detect and quantify mycotoxins. Furthermore, it can display the physiological and pathological effects of mycotoxins.

Adapted from: Food and Beverage Mycology, 2nd Edition, Larry R. Beuchat, 1987

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