This method is quite similar to somatic hybridization. Instead of fusing two cells together, like in somatic hybridization, this technique fuses the protoplast with the added DNA in the culture medium. An electrical pulse is also used in this method. The transformed cell will then reconstruct its cell wall and grow to a whole, fertile plant. However, the inability for most plant species to regenerate from protoplasts makes this method ineffective.
Adapted from: Safety of Genetically Engineered Foods Approaches to Assessing Unintended Health Effects, Institute of Medicine and National Research Council of the National Academies, 2004, The National Academy Press
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